Philips SEM 525 Instructions

Text of Philips SEM 525 User Guide:

• Philips SEM 525, PHILIPS SEM 525 INSTRUCTIONS Start-up: Turn master mains switch on (if not already on) Turn the water cooler/circulator timer on to HOLD (counter clockwise) Press the SEM mains button ON (lower left panel, listen for the mechanical vacuum pump to come on) Press the vacuum ON button The red high vacuum light (left most panel) will be lit. After about 30 minutes, this light indicator will turn off indicating a sufficient vacuum to operate the microscope. However, to ensure maximum filament life, do not turn the beam on

• Use gun bias 1 for 20 and greater kv and bias 2 for 10 – 20 kv. Use a spot size of 500 and the lowest magnification to align the electron gun. Non-conductive samples may charge with these settings. Biological specimens that have been coated usually can be viewed with a lower accelerating voltage, usually 10 – 15 kV. With

• Philips SEM 525, Do not handle specimen stubs with your bare fingers. Contaminates from your fingers will volatilize in the high vacuum and contaminate the final beam aperture and detectors which in turn will significantly degrade the image. The “X” and “Y” controls are approximately centered at 500 X 500 with TILT at 0 degrees. Do not adjust the TILT less than 0 degrees nor greater than 90 degrees. Turning ON the electron beam: Turn ON the high tension. There should be a “blip” in the filament current gauge. Adjust the current to saturation (usually 5-6 clicks). Do not over- saturate the filament. A

• Philips SEM 525, Press and “release” the AIR button and press the vacuum ON button. You should hear the vacuum pull the door sealed shut. When the vacuum is ready (10 or less on the vacuum gauge and RED high vacuum light is off), the electron beam may be turned on. Shutting down the SEM: Turn the filament current to zero. Turn off the high tension by pushing the HT button and letting it release. Turn the black detector gain down 1 turn (clockwise). Turn off the SE detector (push the button and let it release). Turn the spot size to 500 (this is the lowest lens current setting). Turn the magnific

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