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GE UV-1 Operation & User’s Manual

GE UV-1 Manual Online:

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GE UV-1 User Manual
GE UV-1 User Guide
GE UV-1 Online Manual

Text of GE UV-1 User Guide:

  • GE UV-1, 4. Operation 4.3 Conversion T% AU OD OD table T% to AU (3 mm cell) (10 mm cell) and OD 1 5 10 15 20 25 30 32 35 40 45 50 55 60 63 65 70 75 79 80 85 89 90 95 95.5 97.7 2.000 1.301 1.000 0.824 0.699 0.602 0.523 0.500 0.456 0.398 0.347 0.301 0.260 0.222 0.200 0.186 0.155 0.125 0.100 0.097 0.070 0.050 0.046 0.022 0.020 0.010 6.667 4.337 3.333 2.747 2.330 2.007 1.743 1.667 1.520 1.327 1.157 1.003 0.867 0.740 0.667 0.620 0.517 0.417 0.333 0.323 0.233 0.167 0.153 0.073 0.067 0.033 2.000 1.301 1.000 0.824 0.699 0.602 0.523 0.500 0.456 0.398 0.347 0.301 0.260 0.222 0.200 0.186 0.155 0.125 0.100 0.097 0.070 0.050 0.046 0.022 0.

  • GE UV-1, 3. Installation 3. Installation 3.1 Site UV-1 should be installed on a stable, flat surface away from all sour- requirements ces of vibration. The atmosphere should be free of both excess humi- dity and corrosive or contaminated vapours which may form deposits on the component in the optical path. UV-1 can be installed either in a coldroom or at ambient temperature in the laboratory. To minimise drift, the temperature should be kept constant. UV-1 optical unit should be positioned away from all s

  • 2. General Description 2. General Description 2.1 Basic principle GE Healthcare LKB Monitor UV-1 consists of an optical unit containing the flow cell, lamp, filter assemblies and preamplifiers, and a control unit containing the signal processing circuits. The two units are connected via a multi-core cable hardwired from the control unit to the optical unit. Connection to the reco

  • GE UV-1, 5. Maintenance 5.9 6. Use an Allen key (2.5 mm) to unscrew the upper of the two screws securing the lamp holder and the lamp. Note carefully the positions of the insulation sleeve and washer. 7. Disconnect the lamp from the PC-board. 8. Bend the upper part of the lamp holder slightly (2-3 mm) upwards and withdraw the mercury lamp from it by gently pulling the metal socket. 9. Insert the new mercury lamp and connect it to the PC-board. 10. Remount the upper lamp holder screw, ensuring that the insulations

  • GE UV-1, 1. Introduction 1. Introduction The UV-1 is a fixed wavelength UV monitor, consisting of a control unit and an optical unit that can be positioned up to 10 meters apart. A mercury lamp is the stable light source, furthermore a built-in reference cell eliminates baseline drift. The output signal can be recor- ded in either AU or %T. Sensitivity range between 0.01 and 2 AUFS, or (0-100%T). The UV-1 offers a range of three detection wavelengths: 254, 280 and 405 nm. In addition, five flow cells are available for d

  • GE UV-1, Important user information Reading this entire manual is recommended for full understanding and use of this product. ● The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument. Should You have any comments on this manual, we will be pleased to receive them at: GE Healthcare Bio-Sciences AB S-75182 Uppsala Sweden GE Healthcare Bio-Sciences AB reserves the right to changes in the specifications without prior notice. make Warranty and Liability GE Healthcare Bio-S

  • GE UV-1, 3. Installation 3.5 Installation of the flow cell Monitor UV-1 accepts flow cells for Standard Chromatography, FPLC and industrial applications. Product Material of Path Total dead IlluminatedPressure Application Code No. wetted parts length volume volume limit area S-2 Fluoro-plastic, 2 mm 80 µl 2 µl 0.3 MPa Standard 19-4840-02 optical quartz (3 bar) Chromatography HR-10 Fluoro-plastic, 10 mm 24 µl 8.7 µl 1.0 Mpa FPLC, Standard 19-6254-02 optical quartz,titanium (10 bar) Chromatography 3 mm Fluoro-plastic, 3 mm 50 µl 3 µl 1.0 MPa Prepa

  • GE UV-1, 7. Technical Specifications 7. Technical Specifications Wavelength range Lamp Filters Operating modes Full scale ranges Noise Linearity Temperature drift Long term drift Time constant Recorder output Environment Power consumption Power supply, voltage frequency Dimensions (LxWxD) Weight 254 nm, 280 nm and 405 nm Hg lamp: for 254, 280, and 405 nm Life time lamp: 8000 hours converter: 2000 hours Interference. Stray light maximum 0,1 % at 254 nm, maximum 0.8% at 280 nm AU or Transmission 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 AUFS 4 x 10-5 AU peak to peak maximum at 254 nm (dry cell) 2 x 10-4 AU peak to peak (typical at 254 nm in flowing liquid) 5 x 10-4 AU peak to peak (typical

  • GE UV-1, 2. General Description Fig. 2 Block diagram The photocurrent from each detector is amplified in a pre-amplifier before passing to the signal processing circuitry in the control unit. If transmission is to be monitored, the signal passes directly to the low pass filter. If AU is to be monitored, the reference cell light intensity (IR) is compared with the sample cell intensity (IS) to form log (IR/IS) in a logarithmic circuit before passing to the low-pass filter. The signal finally passes to the range selector before being presented to the output terminal. 6

  • Contents 1. Introduction........................................................................................4 2. General Description..........................................................................5 2.1Basic principle.............................................................................5 2.2Optical unit........................................

  • GE UV-1, 2. General Description 2.2 Optical unit Front panel controls Fig. 3. Optical unit. Front panel. No. Item Description 1 Cell holder The complete cell holder is removed by turning the locking (Fig. 4:6) knob on the rear panel 2 Sample inlet Inlet for sample flow 3 Sample outlet Outlet for sample flow 4 Reference inlet Inlet for flowing reference liquid. The reference cell may be operated dry, with static reference liquid 5 Reference outlet Outlet for flowing reference liquid 7 12 3 4 5

  • GE UV-1, 8. Insert the new cell, being careful not to touch either of the optical surfaces. See that the inlet and outlet ports are correctly aligned. 9. Retighten the screw washers with the tool provided. Do not use excessive force. 10. Check for leakage. 11. Replace the black cover. For optimum performance, it is essential that the interference filters are clean and free of any particulate material. Do not touch the inter- ference filters. Should the filter become contaminated with dust,finger prints or oil, proceed as follows: - Carefully take out the filter without touching or scratching the surface. - Use lens cleaning tissue dipped in ethanol to gent

  • GE UV-1, 4. Operation 4.6 Basic operating procedure - AU 4.7 Basic operating procedure - Transmission (%T) 4.8 Shut down 1. 2. 3. 4. 1. 2. 3. 4. 5. 6. 7. 1. Switch AU/ %T to AU (Fig. 5:13). Set the range selector (Fig. 5:12) to SHORT and zero the recorder with the recorder zero control. Set the range selector to 2 and adjust the recorder baseline with the baseline adjust (Fig. 5:14). Set the range selector to the appropriate range and readjust the recorder baseline with the baseline adjust (Fig. 5:14). Only minor adjustment should be necessary. Switch AU to %T (Fig. 5:13). Set the range selector (Fig. 5:12) to SH

  • 6. Trouble-shooting 6. Trouble-shooting The UV-1 Monitor has been designed for trouble-free use. If good chromatographic practice is followed, very little difficulty should be experienced. Clean optical surfaces are essential if low noise levels are to be maintained. The following check list of the most frequent problems is meant to be a guide in trouble-shooting. If the ch

  • GE UV-1, 5. Maintenance Cleaning with detergent: ● 1. Remove the cell holder from the optical unit. 2. Pump undiluted cleaning detergent through the cell for at least 2 hours. 3. Rinse the cell with a) distilled water (100 ml) b) ethanol/distilled water (50% v/v, 100 ml) c) distilled water (100 ml) 4. Replace the cell in the optical unit and reconnect the system to be monitored. Cleaning with chromic acid: 1. Prepare fresh chromic acid by adding concentrated sulphuric acid (100 ml) to a saturated solution of sodium dichromate (3.5 ml). Warning: Chro

  • GE UV-1, 3. Installation 3.6 3.7 Connecting The optical unit may be placed on the bench or mounted on the optical unit laboratory scaffolding. For scaffolding mounting, mount the support rod on the optical unit. The rod may be mounted horizontally or vertically (Fig. 4:9). Tighten the the Allen screw firmly. The optical unit should be placed as close as possible to the column outlet. Connect the cable from the optical unit to the 1 l-pin socket on the back of the control unit (Fig. 6:21). The plug has a snap lock. To remove it, squeez

  • GE UV-1, 2. General Description 2.3 Control unit Front panel controls Fig. 5. Control unit. Front panel. No. Item Description 12 Absorbance range Selector for the desired absorbance range selector 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 AU full scale deflection. In the position SHORT, the signal output terminals are disconnected from the rest of the control circuitry and short-circuited. This position is useful when setting zero on the recorder. 13 Mode switch The AU / % T switch, enables the UV-1 to be used AU or %T to monitor the absorba

  • GE UV-1, 2. General Description Rear panel controls Fig. 6. Control unit. Rear panel. No. Item Description 17 18 19 20 21 22 Mains voltage selector Fuse holder Signal outputs Mains inlet Optical unit inlet Ground terminal Selects mains voltage 110, 130, 220 and 240 V Mains fuses: 1 x 250 mA for 110-130 V 60 Hz 1 x 125 mA for 220-240 V 50 Hz The output signal is a 10 mV DC signal. The terminals are connected to a potentiometric recorder via shielded cables supplied. The UV-1 is normally earthed via the mains ground Inlet for the mains cable Inlet for the optical unit To connect the shield of an output signal cable Note: Do n

  • GE UV-1, 3. Installation Mains voltage Voltage selector setting Fuse 3.4 Installation of the filter 110 V 110 250 mA 130 V 130 250 mA 220-230 V 220 125 mA 240 V 240 125 mA 4. Select the mains cable corresponding to your mains outlet. Discard unwanted mains cable immediately. Connect the instrument to a grounded mains outlet. Note: Do NOT switch on. Note: Special care must be taken when handling interference filters. DO NOT touch the filter surface. The filters should not allowed to come in cont

  • GE UV-1, 4. Operation 4.1 Choice of wavelength 4. Operation The UV-1 can be operated at either 254 nm, 280 nm or 405 nm. The choice of wavelength will depend on the spectral properties of both the eluent and the substances to be detected. Proteins and poly- pep-tides containing aromatic amino acids are usually best detected at 280 and 254 nm. Nucleic acids and poly-nucleotides are usually best detected at 254 nm and ferroproteins i.e. hemoglobins, cytochrome and porphyrin derivatives at 405 nm. 4.2 Choice of AU or The UV-1 may be set to monitor eit

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