Siemens Rapidlab 1200 Operator's Manual
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Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Maintenance 5-9 02087462 Rev. V Performing Every 60 Days Maintenance The every 60 days maintenance schedule is based on analyzing 30 samples per day, unless otherwise noted. If your laboratory analyzes more than 30 samples per day, perform this maintenance more frequently. Replacing the CO-ox Sample Chamber You need a new CO-ox sample chamber to perform this task. BIOHAZARD: Wear personal protective equipment. Use universal precautions. Refer to Appendix A, Protecting Yourself from Biohazards for recommended precautions when working with biohazardous m
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: System Overview and Intended Use 1-41 02087462 Rev. V The potassium sensor membrane is designed as a charge separator. The positively charged potassium ions selectively interact with the membrane when the sample interfaces with the membrane. The negatively charged chloride ions do not interact with the membrane. The charge separation causes the membrane potential measured by the electrochemical cell. Figure 1-34 Illustrated Electrolyte Sensor 1 Internal electrolyte solution 2 Membrane 3 Electrolyte solution 4 Randomly oriented ions
Siemens Rapidlab 1200, 1-64 Rapidlab 1200 Operator’s Guide: System Overview and Intended Use 02087462 Rev. V OBF is the oxygen binding factor. The system uses the default value of 1.39, or whatever value is entered as the default value in Setup.
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Troubleshooting 6-77 02087462 Rev. V CO-ox Chamber Temp Error The system detects that the temperature of the CO-ox sample chamber is beyond the acceptable range. This message occurs when a new sample chamber is warming up or when the system is warming up after being shut down. Results for tHb are reported while this message appears. The system also displays the message on the printed report. Ensure that you installed the CO-ox sample chamber properly. Ensure that you tightly close the front cover. T
5-32 Rapidlab 1200 Operator’s Guide: Maintenance 02087462 Rev. V 4. If you replaced the pH or Na + sensors, condition the sensors. Refer to Conditioning the Sensors‚ page 5-6. 5. Analyze a minimum of 2 levels of quality control material. Filling the Sensor You need the following materials for this task: •pH or Na + , K + , Cl¯, and Ca ++ sensor • appropriate sensor fill solution kit
Rapidlab 1200 Operator’s Guide: Troubleshooting 6-81 02087462 Rev. V No W Cartridge The system does not detect the wash cartridge. The cartridge may not be installed correctly. Reinstall the cartridge. Replace the cartridge. Refer to Replacing the Wash Cartridge‚ page 2-2. Out of Reporting Range The parameter shown in the message is above or below the valid measu
Siemens Rapidlab 1200, E-20 Rapidlab 1200 Operator’s Guide: System Specifications 02087462 Rev.V Recovery and Precision of Blood Gases in Human Whole Blood To prepare samples for analysis, blood was collected in heparinized vacuum tubes and tonometered at 37ºC for each of 6 levels for pH and 9 levels for pCO 2 /pO 2 . Multiple runs were performed using these samples on a minimum of 3 Rapidlab 1245 systems. † Total SD = total standard deviation Table E-17 Rapidlab 1245 AutomaticQC Precision Results Parameter Level n Mean WRSD * Total SD † pH 1 170 7.15 0.002 0.002 pH 2 172 7.352 0.001 0.002 pH 3 168 7.553 0.002 0.003 pCO 2 1 169 68.8 1.18 1.38 pCO 2 2 172 39.1 0.59 0.95 pCO 2
Rapidlab 1200 Operator’s Guide: System Specifications E-41 02087462 Rev.V Table E-39 1260 Recovery and Precision Testing—Cl - Level Mode N WRSD * Observed Expected Recovery WRCV 1Capillary240.58727399.20.8 2Capillary240.29909199.30.3 3Capillary240.58979898.80.6 4 Capillary 24 0.58 104 106 97.8 0.6 5 Capillary 24 0.50 110 111 99.1 0.5 6 Capillary 24 1.08 146 148 98.8 0.7 1 Syr
Siemens Rapidlab 1200, E-70 Rapidlab 1200 Operator’s Guide: System Specifications 02087462 Rev.V Micro- syringe 145 1.071 -0.093 0.068 0.997 0.44 4.67 Table E-72 Rapidlab 1265 Human Whole Blood Method Comparison—Cl - Mode N Slope Intercept RMSE r 2 Min Max Capillary 126 1.055 -4.597 1.382 0.996 70 150 Syringe 265 1.084 -7.862 1.621 0.995 62 151 Micro- capillary 72 0.907 15.580 1.647 0.992 86 145 Micro- syringe 144 0.938 12.479 1.556 0.994 85 154 Table E-73 Rapidlab 1265 Human Whole Blood Method Comparison—Glucose Mode N Slope Intercept RMSE r 2 Min Max Capillary 193 0.896 15.91 25.41 0.987 47 806 Syringe 387 0.899 19.57 31.62 0.981 45 774 Micro- capillary 138 0.879 12.881 23.914 0.
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Maintenance 5-55 02087462 Rev. V Flushing the Tubing for the Measurement Module 1. Disconnect the tubing from the left side of the pressure sensor bubbler. 2. Connect the syringe tubing to the pressure sensor bubbler. 3. Flush the measurement module tubing with a minimum of 3 mL water. 4. Flush the measurement module tubing with a minimum of 12 mL air. 5. Collect the water in an absorbent swab or equivalent. 6. Reconnect the tubing to the pressure sensor bubbler. Figure 5-29 Measurement Module Pressure Sensor Bubbler 1 Pressure sensor bubbler 2 Discon
Siemens Rapidlab 1200, 1-4 Rapidlab 1200 Operator’s Guide: System Overview and Intended Use 02087462 Rev. V AutomaticQC Module The optional AutomaticQC module allows the system to automatically analyze QC materials. The system performs analysis of the AutomaticQC levels at pre-programmed intervals. For information about programming the intervals, refer to Setting up AutomaticQC Schedule‚ page 8-5. Figure 1-3 Rapidlab 1200 System with AutomaticQC Module The AutomaticQC module consists of the following components: • AutomaticQC cartridge • AutomaticQC interface AutomaticQC Cartridge The AutomaticQC cartridge has bags containing 3 levels of quality control material used for verification of perform
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Maintenance 5-39 02087462 Rev. V Maintaining the Internal Reference Electrode 1. Remove the internal reference electrode using the hex tool. Figure 5-20 Reference Sensor Casette 2. If you are replacing the internal reference electrode, dispose of the electrode according to your institution’s protocol. 3. If you are replacing the internal reference electrode, rinse the electrode compartment with KCl fill solution: a. Empty the fill solution from the sensor. b. Rinse the internal electrode comp
Siemens Rapidlab 1200, 2-2 Rapidlab 1200 Operator’s Guide: Operating the System 02087462 Rev. V Replacing Supplies Before you replace supplies, you can view a video of the following procedures: • Emptying the waste bottle • Replacing the wash cartridge • Replacing the reagent cartridge • Replacing the AutomaticQC cartridge Emptying the Waste Bottle The system displays the Waste symbol on the banner when the waste bottle is 70% full. This enables you to empty the bottle at a time when the system is not busy. If you must replace the bottle before you can perform any other tasks, the system displays a message. BIOHAZARD: Wear personal protective equipment. Use
Siemens Rapidlab 1200, 8-46 Rapidlab 1200 Operator’s Guide: System Configuration 02087462 Rev. V Importing and Exporting Maintenance Activities 1. At the Setup screen, select Secured Options. 2. Select the down arrow button and then select Maintenance. If you want to... Then... Import maintenance Data from a disk or CD a. Select Import Schedule. b. Insert a disk or CD in to the drive. c. Select Disk or CD. Schedule information is copied from the disk or CD. d. When prompted, remove the disk or CD from the drive. Export maintenance Data to a disk or CD a. Select Export Schedule b. Insert a disk or CD in to the drive. c. Sele
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Troubleshooting 6-13 02087462 Rev. V The parts listed in the table below are optional, as explained in the Clearing the Clot from the Reagent Manifold procedure below. Clearing the Clot from the Reagent Manifold NOTE: A security access of level 1 or 2 is required to access Diagnostics features. 1. Pull out and lift the front cover of the system. Enter your password, if necessary. 2. Select Status > Diagnostics. Enter your password, if necessary. 3. At the Diagnostics screen, select Cartridges > Eject Both Cartridges. Remove the reagent and wash cartridges. 4. Detach th
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Maintenance 5-11 02087462 Rev. V c. Disconnect the tubing from the connector at the pressure sensor bubbler. 5. Connect the new tubing: a. Connect the new tubing to the spring-loaded latch. b. Push the new tubing into the pinch valve. Center the tubing and ensure the tubing does not have any sharp bends. c. Connect the new tubing to the pressure sensor bubbler. 6. Close the measurement module door. 7. Select No. 8. Reinstall the reagent cartridge and close the reagent cartridge door. 9. Close the front cover. 10. Dispose of the tubing according to your institution’s protocol. Testing for Leaks 1. To ensure that the
Siemens Rapidlab 1200, 2-14 Rapidlab 1200 Operator’s Guide: Operating the System 02087462 Rev. V NOTE: You can scan the patient ID and accession number in the appropriate field. For information about entering patient sample data, refer to Entering Patient Sample Data‚ page 2-22. 8. View the results. For information about how results are displayed on the screen and in printed reports, refer to Viewing Patient Results‚ page 2-27. 9. Select Continue when you finish viewing the results. Analyzing Microsamples NOTE: Read this procedure completely before performing microsample analysis for the first time. Use this procedure to analyze patient samples when you know that you have insufficient sample vo
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: Troubleshooting 6-67 02087462 Rev. V D39 Obstruction D40 W Cartridge Prime Error: 1, 2 Problem Corrective Action The system detects an obstruction while moving the sample for analysis. Correct any reagent problems such as D23 codes before you attempt to correct this D code. • Select Diagnostics > Fluidic Functions > RCx. • Correct any new D23 problems if they occur. • Remove obstructions from reagent manifold, measurement module, CO-ox sample chamber, and sample tubing where you suspect
Siemens Rapidlab 1200, Rapidlab 1200 Operator’s Guide: System Specifications E-69 02087462 Rev.V Micro- capillary 107 1.011 -0.480 3.939 0.996 6.0 232.5 Micro- syringe 216 0.991 -0.772 3.258 0.997 5.9 232.3 Table E-68 Rapidlab 1265 Human Whole Blood Method Comparison—pO 2 Mode N Slope Intercept RMSE r 2 Min Max Capillary 198 0.999 1.888 5.745 0.999 29.2 676.3 Syringe 410 0.979 4.294 8.343 0.998 28.3 683.6 Micro- capillary 107 0.967 3.922 7.086 0.999 26.3 674.4 Micro- syringe 216 0.964 3.089 8.702 0.998 26.4 676.1 Ta
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